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Publication Abstract Display
Type: Published Manuscript
Title: OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis.
Authors: Carlson KA, Limoges J, Pohlman GD, Poluektova LY, Langford D, Masliah E, Ikezu T, Gendelman HE
Contact: Center for Neurovirology and Neurodegenerative Disorders, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5215, USA.
Year: 2004
Publication: Journal of Neuroimmunology
Volume: 150 Issue: 1-2 Pages: 186-98
Abstract:OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.
Funding: NIDA:DA DA12065, NIMH:MH K08MH01552, NIMH:MH MH45294, NIMH:MH MH59745, NIMH:MH P01MH57556, NINDS:NS P01NS31492, NCRR:RR P20RR15635, NIAID:AI R01 AI5089401, NINDS:NS R01NS34239, NINDS:NS R37NS36136
Keywords: AIDS Dementia Complex, Brain, Cells, Cultured, Comparative Study, DNA-Binding Proteins, Disease Progression, Encephalitis, Viral, Gene Expression Regulation, HIV-1, Humans, Macrophages, Organ Specificity, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., Severity of Illness Index, Transcription Factors, Virus Replication

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