| Publication Abstract Display | | Type: Published Manuscript | | Title: HIV-1 neutralizing antibody response and viral genetic diversity characterized with next generation sequencing. | | Authors: Carter CC, Wagner GA, Hightower GK, Caballero G, Phung P, Richman DD, Kosakovsky Pond SL, Smith DM | | Year: 2015 | | Publication: Virology | | Volume: 474 Issue: Pages: 34-40 | | Abstract:To better understand the dynamics of HIV-specific neutralizing antibody (NAb), we examined associations between viral genetic diversity and the NAb response against a multi-subtype panel of heterologous viruses in a well-characterized, therapy-naïve primary infection cohort. Using next generation sequencing (NGS), we computed sequence-based measures of diversity within HIV-1 env, gag and pol, and compared them to NAb breadth and potency as calculated by a neutralization score. Contemporaneous env diversity and the neutralization score were positively correlated (p=0.0033), as were the neutralization score and estimated duration of infection (EDI) (p=0.0038), and env diversity and EDI (p=0.0005). Neither early env diversity nor baseline viral load correlated with future NAb breadth and potency (p>0.05). Taken together, it is unlikely that neutralizing capability in our cohort was conditioned on viral diversity, but rather that env evolution was driven by the level of NAb selective pressure. |
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