Publication Abstract Display
Type: Published Manuscript
Title: Mitochondrial injury and cognitive function in HIV infection and methamphetamine use.
Authors: Var SR, Day TRC, Vitomirov A, Smith DM, Soontornniyomkij V, Moore DJ, Achim CL, Mehta SR, Perez-Santiago J
Year: 2016
Publication: AIDS (London, England)
Volume: 30 Issue: 6 Pages: 839-848
Abstract:In this work we evaluated the association of human immunodeficiency virus (HIV) infection and methamphetamine (METH) use with mitochondrial injury in the brain and its implication on neurocognitive impairment. DESIGN: Mitochondria carry their genome (mtDNA) and play a critical role in cellular processes in the central nervous system. METH is commonly used in HIV-infected populations. HIV infection and METH use can cause damage to mtDNA and lead to neurocognitive morbidity. We evaluated HIV infection and METH use with mitochondrial injury in the brain. METHODS: We obtained white and gray matter from Brodmann's areas (BA) BA7, BA8, BA9, and BA46 of a) HIV-infected individuals with history of past METH use (HIV+METH+, n = 16), b) HIV-infected individuals with no history of past METH use (HIV+METH-, n = 11), and c) HIV-negative controls (HIV-METH-, n = 30). We used the common deletion, a 4,977 bp mutation, as a measurement of mitochondrial injury, and quantified levels of mtDNA and common deletion by droplet digital PCR, and evaluated in relation to neurocognitive functioning (Global Deficit Score [GDS]). RESULTS: Levels of mtDNA and mitochondrial injury were highest in white matter of BA46. A higher relative proportion of mtDNA carrying the common deletion was associated with lower GDS (p < 0.01) in HIV+METH+ but higher GDS (p < 0.01) in HIV+METH-. CONCLUSIONS: Increased mitochondrial injury was associated with worse neurocognitive function in HIV+METH- individuals. Among HIV+METH+ individuals, an opposite effect was seen.

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