Publication Abstract Display
Type: Published Manuscript
Title: Replication of macrophage-tropic and T-cell-tropic strains of Human Immunodeficiency Virus Type 1 is augmented by macrophage-endothelial cell contact.
Authors: Gilles PN, Lathey JL, Spector SA
Contact: Department of Pediatrics, University of California, San Diego, La Jolla 92093-0672.
Year: 1995
Publication: Journal of Virology
Volume: 69 Issue: 4 Pages: 2133-9
Abstract:Macrophages perform a central role in the pathogenesis of human immunodeficiency virus type 1 (HIV-1) infection and have been implicated as the cell type most prominent in the development of central nervous system impairment. In this study, we evaluated the effect of interaction between macrophages and endothelial cells on HIV-1 replication. Upregulation of HIV-1 replication was consistently observed in monocyte-derived macrophages (hereafter called macrophages) cocultured with either umbilical vein endothelial cells or brain microvascular endothelial cells. HIV-1 p24 antigen production of laboratory-adapted strains and patient-derived isolates was increased 2- to 1,000-fold in macrophage-endothelial cocultures, with little or no detectable replication in cultures containing endothelial cells only. The upregulation of HIV-1 in macrophage-endothelial cocultures was observed not only for viruses with the non-syncytium-inducing, macrophage-tropic phenotype but also for viruses previously characterized as syncytium inducing and T-cell tropic. In contrast, cocultures of macrophages with glioblastoma, astrocytoma, cortical neuronal, fibroblast, and placental cells failed to increase HIV-1 replication. Enhancement of HIV-1 replication in macrophage-endothelial cocultures required cell-to-cell contact; conditioned media from endothelial cells or macrophage-endothelial cocultures failed to augment HIV-1 replication in macrophages. Additionally, antibody to leukocyte function-associated antigen (LFA-1), a macrophage-endothelial cell adhesion molecule, inhibited the enhanced HIV-1 replication in macrophage-endothelial cell cocultures. Thus, these data indicate that macrophage-endothelial cell contact enhances HIV-1 replication in macrophages for both macrophage-tropic and previously characterized T-cell-tropic strains and that antibody against LFA-1 can block the necessary cell-to-cell interaction required for the observed upregulation. These findings may have important implications for understanding the ability of HIV-1 to replicate efficiently in tissue macrophages, including those in the brain and at the blood-brain barrier.
Funding: NIAID:AI AI-128270, NIAID:AI AI-272670, NIMH:MH MH-45294
Keywords: Antibodies, Monoclonal, Brain, Cells, Cultured, Endothelium, Vascular, HIV-1, Humans, Lymphocyte Function-Associated Antigen-1, Macrophages, Membrane Fusion, Research Support, U.S. Gov't, P.H.S., Skin, T-Lymphocytes, Tumor Cells, Cultured, Virus Replication

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