| Publication Abstract Display | | Type: Published Manuscript | | Title: Human Immunodeficiency Virus DNA is present in a high percentage of CD4+ lymphocytes of seropositive individuals. | | Authors: Hsia K, Spector SA | | Contact: Department of Pediatrics, University of California, San Diego 92103. | | Year: 1991 | | Publication: The Journal of Infectious Diseases | | Volume: 164 Issue: 3 Pages: 470-5 | | Abstract:Human immunodeficiency virus type 1 (HIV-1) infects predominantly CD4+ cells in human peripheral blood and infection is associated with CD4+ lymphocyte dysfunction in patients with AIDS. To determine the frequency of HIV-1 infection in CD4+ lymphocytes in vivo, peripheral blood CD4+ lymphocytes were isolated by fluorescence-activated cell sorting from HIV-1-infected persons with clinical disease ranging from asymptomatic to AIDS. Using standard and booster polymerase chain reaction analyses, study patients with AIDS and AIDS-related complex (ARC) were found to harbor the HIV-1 genome in at least 10% of CD4+ lymphocytes, and approximately 10-fold less infected cells were found in those with asymptomatic infection. In addition, the peripheral blood mononuclear cells from patients with ARC frequently contained a higher absolute number of HIV-1-infected CD4+ lymphocytes than those with AIDS or asymptomatic infection. It is likely that this high level of infection of CD4+ lymphocytes is the primary cause for the progressive immunologic deficiency observed in patients infected with HIV-1. | | Funding: NIAID:AI AI-27563, NIAID:AI AI-27670, NHLBI:HB HB-67019 | | Keywords: AIDS-Related Complex, Acquired Immunodeficiency Syndrome, CD4-Positive T-Lymphocytes, Cell Line, DNA, Viral, HIV Seropositivity, HIV-1, Humans, Polymerase Chain Reaction, Research Support, U.S. Gov't, P.H.S. |
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